Lactobacillus acidophilus (SD-5221) is the lead active here, studied most for comfort after dairy. In the 100 Billion CFU Replenish Blend with 32 others, listed second. Cherry (Fruit) is the most studied active here, with 21 human studies behind it, each one cited. Who it fits: for everyone, adults.
Verified by IngredientMDThe panel on the left is the one we evaluated, line by line.
All 46 actives checked against the clinical evidence: dose, form, and what the research actually shows.
Doses, ratios, and how the ingredients work together. That assessment is the verdict above: "Backed by human trials".
The verdict is public and identical whether or not a brand pays. A formula that does not clear the bar gets nothing.
Read by the engine against 2.1 million data points from clinical trials, PubMed and more than a hundred vetted sources. No individual reviewer is named or implied.
Cross-checked against 172 documented interactions for these actives.
Public health figures the engine matched to this formula's ingredients. Reported by their source, cited and dated, never written by the brand.
Randomised trial, 2026
Strain LB42 was assessed from preclinical safety through to gut microbiota and immune measures, reporting effects on microbial composition and immune markers.
Food and Chemical ToxicologyMeta-analysis, 2026
Pooling trials of probiotics in healthy people, probiotics shifted gut microbe makeup, with only small changes in overall microbial diversity.
BMC medicineRandomised trial, 2025
Regular sauerkraut intake, a food fermented largely by Leuconostoc mesenteroides, produced modest and mostly short-lived shifts in the gut microbial community of healthy adults.
MicrobiomeRandomised trial, 2026
A combination of Lactiplantibacillus plantarum Q180 with a microalgal component was assessed against control, with tolerability recorded alongside the primary measures.
NutrientsRandomised trial, 2025
In healthy adults, polyphenols from raspberry leaf tea lowered the post-meal glucose and insulin rise compared with control.
NutrientsSystematic review, 2025
Reviewing human trials, strains including L. helveticus were linked to modest improvements in self-reported mood, with gut metabolite production put forward as the mechanism.
International journal of molecular sciencesRandomised trial, 2026
Heat-killed Lactobacillus acidophilus IDCC 3302 improved measured skin hydration and wrinkle parameters compared with placebo over the supplementation period.
NutrientsRandomised trial, 2026
Lactobacillus paracasei NB23 changed IGF-1 levels, muscle function measures and cardiometabolic risk markers in healthy participants.
Probiotics and Antimicrobial ProteinsRandomised trial, 2025
In a trial run around a marathon, probiotics shifted blood inflammation markers and self-reported sleep, a marker and a self-reported measure rather than a measured outcome.
NutrientsRandomised trial, 2024
Daily supplementation with carrot-derived rhamnogalacturonan-I altered gut microbiota composition in healthy adults. Composition is a measured community change, not a clinical outcome.
Biomedicine and PharmacotherapySystematic review, 2020
The review of probiotic organisms, including Saccharomyces strains, concluded that certainty of the evidence for the outcomes assessed was low and that effects were inconsistent across trials.
Cochrane Database of Systematic ReviewsRandomised trial, 2026
A two-strain preparation containing Lactiplantibacillus plantarum was assessed against control in the source's trial, with outcomes reported as laboratory and questionnaire measures.
NutrientsSystematic review, 2025
Fermented foods carrying Lactobacillus or Bifidobacterium were linked with improvements in gut microbial and metabolic markers across the human studies reviewed, with effects varying by strain and food.
Frontiers in nutritionRandomised trial, 2024
Reports glycaemic index, lipid panel and microbiome composition measures in adults supplemented with Lactobacillus rhamnosus. All three are laboratory markers rather than clinical outcomes.
FoodsRandomised trial, 2026
Thirty days of eating selenium-enriched strawberries shifted blood sugar handling and liver enzyme readings versus a control group, though biofortification means selenium may explain only part of the change.
NutrientsSystematic review, 2026
Across the pooled literature, Lactobacillus and Bifidobacterium species showed effects on glucose metabolism measures that varied considerably by strain and study.
Nutrition ReviewsRandomised trial, 2015
An acute crossover trial of a multi-ingredient product reporting changes in substrate utilisation and perceived hunger markers. Pepper-derived constituents appear as one component of the blend, so the result cannot be assigned to green bell pepper.
Journal of the International Society of Sports NutritionRandomised trial, 2024
The authors report effects of a defined Lactiplantibacillus plantarum KC3 and Leonurus japonicus combination against placebo on their prespecified respiratory comfort measures.
NutrientsRandomised trial, 2026
A synbiotic combining strains including this species with fibre was compared against control for abdominal visceral fat measures and gut microbiota in adults carrying excess body weight. The intervention was a multi-component synbiotic, so nothing is attributable to one strain.
Clinical NutritionRandomised trial, 2025
A trial of a fermented probiotic food tested alcohol intake and next-day symptoms, with Leuconostoc mesenteroides named as part of the broader product, not tested on its own.
NutrientsRandomised trial, 2026
Evaluates a preparation containing Lactobacillus rhamnosus across clinical, biological and patient-reported measures in a single trial. The multidimensional design is the paper's contribution.
NutrientsRandomised trial, 2026
Combined probiotic and iron-folic acid supplementation was assessed for iron status and gut inflammation markers. Both are laboratory markers and the probiotic component was one part of a combined regimen.
European Journal of Clinical NutritionSystematic review, 2023
Combining earlier reviews, probiotics raised one blood antioxidant marker and lowered another marker of oxidative balance, not outcomes on their own.
Frontiers in nutritionRandomised trial, 2025
Supplementation with Lactobacillus helveticus plus Bifidobacterium longum was reported to change hormonal and related laboratory measures relative to control. These are markers, not clinical outcomes.
Nutrition JournalRandomised trial, 2025
A multi-species synbiotic increased measured gut microbial diversity and the production of urolithin A and butyrate, which the authors report as microbial markers rather than clinical endpoints.
NutrientsOpen-label trial, 2025
A study of a probiotic strain delivered on oat flakes measured blood and urine markers rather than health outcomes, with the oat delivery method part of what was tested.
MedicinaOpen-label trial, 2025
A small pilot study of black raspberry supplementation in older adults with excess body weight reported changes in the measured markers. The authors present it as preliminary and hypothesis-generating.
npj Science of FoodMeta-analysis, 2024
Pooling nine randomized trials in 558 adults, ashwagandha lowered perceived stress scores by about 4.7 points and serum cortisol by about 2.6 micrograms per deciliter compared with placebo.
ExploreMeta-analysis, 2021
Across five randomized trials in 400 adults, ashwagandha extract improved overall sleep with a small pooled effect (standardized mean difference -0.59), most noticeable at doses of 600 mg or more per day taken for at least eight weeks.
PLoS OneMeta-analysis, 2020
Pooling four randomized trials in 142 athletes and healthy adults, ashwagandha raised maximal oxygen uptake (VO2max) by about 3.0 mL/kg/min compared with placebo.
NutrientsRandomised trial, 2025
The authors report that a bioconversion-based postbiotic preparation was associated with changes in muscle strength measures and in gut microbiota composition in healthy adults over the trial period.
NutrientsRandomised trial, 2025
Pre-sleep casein with probiotic strains was associated with changes in anaerobic power and lower-body strength measures compared with casein alone.
Journal of the International Society of Sports NutritionRandomised trial, 2023
In a controlled trial, daily strawberry powder was linked to better memory test scores and lower self-reported mood symptom scores, both measured test results rather than clinical outcomes.
NutrientsRandomised trial, 2018
In older women with lower bone density, 12 weeks of annatto tocotrienol lowered blood markers of bone breakdown and oxidative stress relative to placebo. These are markers, not measured bone outcomes.
The paperMeta-analysis, 2022
Pooling trials, ashwagandha lowered self-reported anxiety and stress scores compared with placebo.
Phytotherapy researchCohort study, 2026
Culture-based sampling described which intestinal lactobacilli were recoverable in young people compared with centenarians. This is an association across age groups and not a supplementation effect.
Frontiers in MicrobiologyRandomised trial, 2025
Daily strawberry intake was associated with modest gains on some cognitive tests and small changes in cardiovascular measures in the older adults studied.
Nutrition, metabolism, and cardiovascular disSystematic review, 2026
The review surveys neurobiological effects of microbial interventions in psychiatry and finds the evidence base heterogeneous and preliminary.
Frontiers in PsychiatryRandomised trial, 2025
A randomised controlled trial of a probiotic product containing L. helveticus. The strain was given inside a combination, so no result can be attributed to it alone.
International Journal of Molecular SciencesRandomised trial, 2022
A randomised study of a mixture containing L. helveticus, B. longum and Lactiplantibacillus. The mixture is the unit that was tested.
NutrientsMeta-analysis, 2019
Across combined trials, strawberries improved several heart-related blood markers in adults, including lower LDL cholesterol and a blood marker of inflammation.
Food & functionSystematic review, 2024
Across the reviewed human studies, higher dietary carotenoid intake tracked with more favourable cardiovascular risk markers, an association rather than a demonstrated cause.
NutrientsMeta-analysis, 2026
Pooled trials reported modestly lower circulating cortisol with probiotic supplementation, with the certainty of the pooled estimate graded as limited.
BMC nutritionSystematic review, 2025
Pooled trials reported higher blood levels of brain-derived neurotrophic factor, a signalling marker, after probiotic supplementation, with the effect graded as small and dose dependent.
BMC nutritionOpen-label trial, 2025
Black raspberry lozenges were associated with lower benzo[a]pyrene DNA adduct levels in buccal cells of adults who smoke. An exposure biomarker, not a health outcome.
CarcinogenesisRandomised trial, 2026
A 12-week double-blind placebo-controlled exploratory pilot of a heat-treated Bifidobacterium preparation reported pilot-scale findings the authors describe as exploratory and needing confirmation.
BMC NutritionRandomised trial, 2022
A low glycaemic index energy-restricted diet changed faecal short-chain fatty acid concentrations, while adding Lactobacillus rhamnosus did not produce a detectable additional change. A failure to detect a difference, not evidence that none exists.
European Review for Medical and Pharmacological SciencesRandomised trial, 2018
Over 12 weeks in older women, annatto-derived tocotrienol was well tolerated, and the trial did not detect a difference in quality of life measures compared with placebo.
BMC complementary and alternative medicineMeta-analysis, 2024
Pooled trials of L. plantarum supplementation reported changes in glucose and lipid laboratory measures in adults with high blood sugar, with the authors noting heterogeneity between studies.
Clinical Nutrition ESPENRandomised trial, 2026
Infants given a specific probiotic strain had fewer caregiver-reported bouts of skin irritation and breathing or digestive symptoms than infants without it, specific to that strain and dose.
QJMRandomised trial, 2026
A randomised trial of Lactobacillus acidophilus in infants assessed both crying behaviour and microbiota composition, with microbiota modulation reported as a co-primary measure alongside the behavioural outcome.
BMC PediatricsRandomised trial, 2026
In early childhood, a different specific probiotic strain was linked to fewer reported bouts of skin irritation and breathing symptoms than no supplement, specific to that strain.
Frontiers in NutritionRandomised trial, 2026
Bifidobacterium animalis subsp. lactis XLTG11 given with a Lactobacillus plantarum strain shifted gut microbiota composition relative to control. Community composition is a measured microbial readout and not a health endpoint.
European Journal of NutritionSystematic review, 2026
The review found strain-specific differences in probiotic effects on dental caries measures in children and adolescents.
Clinical Oral InvestigationsSystematic review, 2025
The authors reviewed probiotic supplementation and infectious and gastrointestinal complications in critically ill patients, reporting mixed results across strains.
Clinical Nutrition ESPENRandomised trial, 2025
A probiotic intervention was evaluated against pulmonary, gastrointestinal and growth measures in a paediatric population, with mixed results across endpoints.
BMC PediatricsRandomised trial, 2026
A randomised trial of early postoperative synbiotic supplementation, with Lactobacillus rhamnosus among the strains, reporting gastrointestinal tolerance measures after bariatric surgery.
Clinical NutritionRandomised trial, 2025
Twelve weeks of preoperative multi-strain probiotic supplementation was compared against placebo on inflammation and metabolic markers.
Nutrition JournalSystematic review, 2026
The review concluded that early-life probiotic effects on respiratory infection measures are strain-specific and cannot be generalised across products.
NutrientsMeta-analysis, 2023
Pooled trials of a probiotic strain in infants reported measurable effects on the outcomes tracked, along with acceptable tolerability.
FoodsPopulation figures from public health data. Context for the category, not a statement about any individual and not a claim about this product.
Each line below was derived from this formula's own record at the time of verification. Nothing here was written by the brand, and nothing can be added that the evidence does not carry.
Time-to-effect windows the engine read from the trial record. Each is a window a named study measured, cited and dated, never written by the brand.
In a randomised preliminary trial, sixteen healthy national-level cross-country skiers took Bifidobacterium lactis BL-99 daily for 8 weeks. Over the supplementation period, gut abundance of Bifidobacterium animalis rose about forty-fold and blood triglyceride levels shifted.
In a double blind trial in 16 recreational runners, 4 weeks of daily Lactobacillus plantarum PS128 capsules taken before a half marathon preserved knee extensor torque and electromyographic measures of muscle function across the 96 hours after the race compared with placebo.
In a double blind, randomized, placebo controlled trial in adults aged 20 to 40 with above-normal body weight, daily Lactobacillus plantarum GKM3 at 1 g per day for 4 weeks increased bowel movement frequency compared with placebo.
In a 60 day randomised, double-blind, placebo-controlled trial, 60 stressed healthy adults took 240 mg of a standardised ashwagandha extract (Shoden) once daily or placebo. The ashwagandha arm showed a larger fall in Hamilton Anxiety Rating Scale scores (p = .040) and in morning cortisol (p < .001) than placebo, with the DASS-21 change near-significant (p = .096). A separate 8 week randomised, double-blind, placebo-controlled dose-ranging trial enrolled 131 adults with self-reported high stress and analysed 98, measuring reduced Perceived Stress Scale scores at 125, 250 and 500 mg per day of an aqueous root and leaf extract. Two trials on two different extracts, both landing in the same eight to nine week band.
Windows measured in the cited trials. Context for how the active behaves over time, not a statement about any individual and not a claim about this product.
12 ingredients, from each one's own page.
Give it one to four weeks of daily use. Stool regularity and less day-to-day bloating are usually the first things people notice.
Digestive changes usually appear within one to two weeks. A steadier bowel pattern generally settles around week three or four.
Digestive changes usually settle in over one to two weeks of daily intake. There is no same day effect to expect from this one.
No published timeline sits behind this particular concentrate. Joint comfort ingredients are generally read over weeks of daily use rather than in a day.
Nothing immediate. Taken daily through the darker months it works in the background, and the difference reads across a season rather than on a single day.
Digestive changes usually show up somewhere between one and four weeks of daily use. Trials that looked at stress and mood measures read them at four weeks or later.
Probiotic changes in digestion usually show over one to four weeks of daily use rather than on the first day.
Easier digestion of a dairy meal happens during that meal, because the enzyme works there. Anything broader is a slow shift measured over weeks.
Digestive changes usually land in the first one to two weeks. Immune measures in trials of blends like this are tracked across eight to twelve weeks.
What ME-3 was studied for is redox and lipid readings on a blood panel, so a repeat test tells you more than how your day feels.
Is the window the stress and mood trials ran on. Digestive changes tend to arrive in the same stretch.
Most people notice digestive changes over one to two weeks. Immune related measures in trials are read after eight to twelve weeks of continuous use.
How each ingredient usually behaves over time, from its own page on IngredientMD. General timing for the ingredient, not a measurement of this product.
It is a capsule, so the amount per serving is fixed and travels without measuring.
Put an earned verification on your own product page.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. This record is not medical advice. Always consult your healthcare provider before starting any supplement regimen.
