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Ingredients/Mushroom/Shiitake Lentinan

Shiitake Lentinan.

Strength pending.The research strength is not set yet.

Common culinary mushroom with serious immune benefits. The characterised beta-glucan fraction of shiitake, purified out of the mushroom. It is recognised by receptors on innate immune cells rather than acting like something you feel.

500 to 2,000mgDaily amount

Reviewed March 2026

SLMushroom
Shiitake LentinanIngredientMD
Category
Mushroom

Also filed under
Immune enhancementCancer adjunctCholesterol

What Shiitake Lentinan is, and what it does.

Does it work
Suits people who want the named glucan fraction with a beta-glucan figure on the label. Dried shiitake carries the same polysaccharide if you would rather get it from food.
How much to take
Start with 100mg to 500mg a day of extract standardised to beta-glucan. Read the beta-glucan number, since a plain polysaccharide figure can be largely substrate starch.
Time to feel it
Weeks. Immune measures in studies move across roughly four to eight weeks of daily use, and that is where a change is read.
The first dose
Day one is quiet. The glucan is not cleaved by human enzymes, so it travels intact and meets immune tissue in the gut wall without registering as a sensation.
With regular use
Weeks of daily use. What people describe is a steadier run through a season rather than an effect that arrives on any particular day.
How well tolerated
Well tolerated by mouth. Check first if you take immune-modulating medicines, and note that raw shiitake causes a temporary skin reaction in some people.
How it feels
Not much to feel. It works at the level of immune cell signalling, so the read-out is how a winter goes rather than a daily sensation.
The overlooked benefit
The clinical record that made lentinan famous used injected preparations. An oral capsule is a different route, which is why the beta-glucan figure carries the weight.

500 to 2,000mg a day is where Shiitake Lentinan works.

How much to take a dayLimited data
500 to 2,000mg
Daily maintenanceThe everyday amount, and where most daily supplements sit. This is the one you take month after month.
5,000mgClinical territory. Trials run high on purpose, for a set number of weeks, against one measured outcome. Impressive to hit, and not what a daily product is for.
Above 8,000mgPast what the research covers. More capsules rather than more effect.
MORE EFFECT ↑02,000mg5,000mg plateauDAILY DOSE →
The shaded band is where the dosing trials landed.

Source: Dai X et al. J Am Coll Nutr. 2015;34(6):478-487

The proof, claim by claim.

These words describe the research, not the molecule's worth. Research strength is how much work stands behind one claim, and it is never a product score.

Shiitake Lentinan has emerging evidence. Based on 7+ studies.

  • innate immune signalling through dectin-1 and complement receptor 3In vitro study
  • immune cell measures with injected preparationsRandomised trial
  • colonic fermentation of unabsorbed polysaccharideNarrative review
  • triple-helix conformation and receptor recognitionIn vitro study
PubMedCochraneClinicalTrials.govNIH ODSSUPP.AILabs test. IngredientMD verifies.PubMedCochraneClinicalTrials.govNIH ODSSUPP.AILabs test. IngredientMD verifies.

Questions people ask about Shiitake Lentinan.

When should I take it?
Timing matters less than consistency. Pick a time that works for you and take it daily.
Can I take it with other supplements?
Usually fine. The main thing to watch is not doubling up on the same ingredient from different products. If you're on prescription meds, check with your pharmacist first.
Any side effects to watch for?
Most people tolerate it well at recommended doses. GI upset is the most common complaint with any supplement. Start with a lower dose and work up. If something feels off, stop and reassess.
Pairs well with21 on file

Why these belong in the same formula. Each row says what the basis is, from settled biochemistry through to a trial that measured the pair.

Lentinan is the purified beta-1,3-glucan with beta-1,6 branches isolated from shiitake, so a lentinan dose and a whole shiitake extract carry the same active fraction at different purity.

Lentinan and yeast beta-glucan are both dectin-1 ligands with the same 1,3 backbone and 1,6 branching motif, so they engage the identical receptor rather than two different ones.

Lentinan is a beta-glucan, so a generic beta-glucan dose alongside it adds more of the same ligand rather than a second mechanism.

Shiitake Lentinan + Maitake (Grifola frondosa)complementary glucan structures

Maitake glucans carry a different branching frequency from lentinan, so a blend presents a wider set of shapes to the same receptor family.

Shiitake Lentinan + Turkey Tail (Trametes versicolor)complementary polysaccharide fractions

Turkey tail supplies protein-bound polysaccharide, a presentation that engages innate immune receptors differently from free lentinan.

Shiitake Lentinan + Vitamin D3 (Immune)cofactor for the same cells

The macrophages and dendritic cells that recognise lentinan through dectin-1 also express the vitamin D receptor, and their response depends on vitamin D status.

Zinc-dependent transcription factors sit downstream of the signalling lentinan triggers, so normal lymphocyte function needs the mineral present.

Shiitake Lentinan + astragalusTraditional pairing in East Asian formulas plus a shared polysaccharide-fraction rationale.

Astragalus and shiitake are both used for their polysaccharide fractions and appear together in traditional formulas. Each has its own preclinical immune-signalling literature. No combination trial supports the pairing, so the label stays early.

Shiitake Lentinan + reishiShared beta-glucan chemistry: both are fungal 1,3-beta-glucans with 1,6 branching.

Lentinan and reishi polysaccharides are recognised by the same pattern-recognition receptors, principally dectin-1 on innate immune cells. Combining them is a way of covering a broader range of chain lengths and branching patterns rather than adding a new mechanism. The receptor biology is well described; the benefit of mixing sources is not measured.

Shiitake Lentinan + cordycepsFormulation practice in mushroom blends with distinct described polysaccharide and nucleoside fractions.

Cordyceps is used for its nucleoside and polysaccharide content, shiitake for lentinan specifically. Blends combine them to widen the constituent profile of a single capsule. There is no combination evidence, and blends often dilute each component.

Shiitake Lentinan + lions-maneFormulation practice in mushroom blends; the two target different described areas.

Lion's mane is included for hericenones and erinacines, shiitake for its beta-glucan fraction. They are stacked because the interests do not overlap, not because they interact. Confidence is early and should stay that way.

Shiitake Lentinan + seleniumEstablished cofactor biochemistry: selenium is required for glutathione peroxidase and thioredoxin reductase activity in immune cells.

Immune cell function depends on selenoenzyme-supported redox control, and selenium status sets how much of those enzymes a person can make. Shiitake is also a modest dietary selenium source. The cofactor relationship is textbook biochemistry and needs no combination trial.

Shiitake Lentinan + vitamin-cEstablished roles of ascorbate in neutrophil function and as a water-phase antioxidant.

Ascorbate accumulates in leukocytes at high concentration and supports normal neutrophil chemotaxis and oxidative burst control. It works in the aqueous phase while a beta-glucan works at the receptor level, so the two do not overlap. Established nutrient biochemistry, not a tested pair.

Shiitake Lentinan + elderberryFormulation practice in seasonal immune blends.

Elderberry anthocyanins and fungal beta-glucans sit in the same category of product for different reasons. The pairing is commercial and traditional rather than mechanistically linked. Nothing has measured the two together.

Shiitake Lentinan + propolisTraditional pairing in immune-support formulas.

Propolis contributes flavonoids and phenolic acids, shiitake contributes a polysaccharide. They are combined by convention. Confidence is early and the rationale is compositional breadth.

Shiitake Lentinan + probioticsEstablished fermentability: beta-glucans and other fungal polysaccharides are substrates for colonic bacteria.

Much of an oral beta-glucan is not absorbed intact and reaches the colon, where it is fermented. That makes a fungal polysaccharide a plausible substrate alongside a live culture. Fermentability is established; which strains benefit and by how much is not settled.

Shiitake Lentinan + inulinEstablished prebiotic fermentation of non-digestible polysaccharides.

Inulin and fungal beta-glucans are both fermented in the colon to short-chain fatty acids, though at different rates and by different organisms. Combining substrates broadens which bacteria are fed. The fermentation chemistry is established; a combined effect on any immune measure is not.

Shiitake Lentinan + lactoferrinEstablished innate immune protein biochemistry: lactoferrin sequesters iron and binds bacterial lipopolysaccharide.

Lactoferrin works by iron withholding and direct binding at mucosal surfaces, a different route from beta-glucan receptor engagement. The two are combined in immune formulas on that logic. Each mechanism is well described; the pairing is not measured.

Shiitake Lentinan + quercetinEstablished flavonoid antioxidant and signalling chemistry.

Quercetin acts on inflammatory signalling and as a radical scavenger, lentinan acts through innate immune receptor recognition. They occupy separate points of the same broad response. This is complementarity by mechanism rather than a demonstrated combination.

Shiitake Lentinan + colostrum-bovineFormulation practice combining a fungal polysaccharide with dietary immunoglobulin.

Colostrum contributes immunoglobulins and growth factors that act at the gut surface. Lentinan is a polysaccharide recognised by innate immune receptors. Products pair them for breadth; no combination data exist.

Shiitake Lentinan + beta-glucan-oatEstablished structural difference: oat beta-glucan is a linear 1,3 and 1,4 glucan, fungal beta-glucan is 1,3 with 1,6 branches.

The two are not the same molecule despite sharing a name. Cereal beta-glucan is viscous and acts mainly in the gut lumen on cholesterol and glucose handling, while branched fungal beta-glucan is the form recognised by dectin-1. Combining them gives two different behaviours, and a label that says only 'beta-glucan' hides which one is present.

Who should be cautious

Nothing specific on file for Shiitake Lentinan. Match the label to the daily amount above, and tell your doctor what you take.

Not medical advice. Show the label to your pharmacist.

What Shiitake Lentinan actually does.

Established

Lentinan is a beta-1,3-glucan with beta-1,6 branches isolated from Lentinula edodes; the branching pattern and triple-helix conformation are what distinguish it from linear cereal beta-glucans.

Established

Branched fungal beta-glucans are recognised by dectin-1 and complement receptor 3 on innate immune cells, a pattern-recognition relationship described extensively in cell and animal work.

Established

Lentinan is a high molecular weight polysaccharide with low oral bioavailability as an intact molecule; the clinical literature that established it used injected preparations, while supplements are oral, and the two routes are not interchangeable.

Established

Hot water is the standard solvent for extracting fungal beta-glucans because the glucan fraction is water-soluble while chitin in the cell wall is not; alcohol precipitation then concentrates the polysaccharide.

More than one route, 6 steps on record

Where Shiitake Lentinan comes from.

The mushroom is grown, dried and simmered in hot water, which pulls out the long-chain sugar that lentinan is. Alcohol is then added to make that sugar drop out of solution so it can be collected and dried. The number worth reading on the label is beta-glucan measured on its own, because a plain polysaccharide figure can be mostly starch from the grain the fungus was grown on.

The same molecule is reached more than one way. Which route a given product used is a manufacturing choice, and the finished compound is the same either way.

Starts as
Lentinula edodes fruiting bodies or mycelium

Fruiting bodies are grown on hardwood logs or supplemented sawdust blocks. Mycelium is grown either on sterilised grain or in submerged liquid culture. These are genuinely different starting materials and the label should say which one was used.

Converted by
Drying

Fruiting bodies are dried before extraction. Ultraviolet exposure during drying converts ergosterol to vitamin D2, which is why some shiitake products carry a vitamin D2 figure and others do not.

Extracted by
Hot water extraction

Water-soluble beta-glucans are pulled out at elevated temperature. Chitin and other insoluble wall material stays behind. Some processes add an alkaline extraction step to release more tightly bound glucan.

Purified by
Alcohol precipitation

Ethanol is added to the aqueous extract to precipitate high molecular weight polysaccharide while leaving small molecules and sugars in solution. Repeated cycles raise the glucan share.

Standardised to
Beta-glucan assay

An enzymatic assay measures beta-glucan specifically and subtracts alpha-glucan starch. This distinction is the whole game on a mushroom label, since a total polysaccharide figure can be mostly starch from a grain substrate.

Ends up as
Spray-dried powder

Dried and blended into capsules, tablets or powders. The oral form is what supplements use; the clinical literature that made lentinan known used an injected preparation under medical supervision, which is a different route entirely.

Whether the material is fruiting body or grain-grown mycelium is frequently not stated, and the two carry different amounts of beta-glucan.

Getting Shiitake Lentinan from food.

The whole-food sources on file. A supplement closes the gap, it does not replace dinner.

Lentinula edodes fruiting bodyDried shiitake mushroomFresh shiitake mushroom

A gram-for-gram figure (how much of each you would eat to match a dose) will appear here once it is sourced and reviewed. This page will not print a number it cannot cite.

The forms it comes in.

Hot water extract standardised to beta-glucanFruiting bodies extracted in hot water, alcohol precipitated, and assayed for beta-glucan by an enzymatic method that separates beta-glucan from alpha-glucan starch.Fits Products where the beta-glucan figure is the reason for inclusion and the label names the assay basis.Trade-off An unqualified polysaccharide percentage can include starch, so the number is only meaningful when beta-glucan is measured specifically.
Purified lentinan fractionFurther chromatographic purification of the hot water extract toward the defined lentinan polysaccharide rather than a mixed glucan fraction.Fits Formulas that name lentinan specifically rather than shiitake extract generally.Trade-off More processing steps and a narrower constituent profile than a whole extract, and purity claims are difficult for a buyer to check.
What the strongest studies found

The essence, in one line each.

  1. Healthy adults took a shiitake-derived beta-glucan against placebo and self-reported quality of life measures were tracked across the supplementation period.Randomised trial. Aldwinckle et al., 2020 (International journal of medicinal mushrooms). PMID 32749096
  2. A soluble beta-glucan taken from shiitake was tested in healthy adults, with immune and wellbeing measures compared against placebo.Randomised trial. Gaullier et al., 2011 (International journal of medicinal mushrooms). PMID 22164761
  3. The trial examined whether orally applied preparations containing non-digestible polysaccharides change the antibody response to influenza vaccination in healthy older adults; lentinan is named within the polysaccharide class rather than tested on its own.Randomised trial. Laue C et al., 2021 (Nutrients). PMID 34444843
  4. A review of food-derived phytochemicals and their described actions on oxidative stress and immune response, in which mushroom polysaccharides including lentinan are named among the compound classes discussed.Narrative review. Gliozheni E et al., 2025 (International Journal of Molecular Sciences). PMID 40806447

These are the studies our verdict leans on, chosen from the 197 we read for Shiitake Lentinan. The full linked list is below.

FDA Disclaimer: These statements have not been evaluated by the Food and Drug Administration. This information is for educational purposes only and is not intended to diagnose, treat, cure, or prevent any disease. Consult your healthcare provider before starting any supplement regimen.